ABSTRACT
Objective To compare the diversity of mitochondrial genomes of Angiostrongylus cantonensis in the mainland of China. Methods According to the population genetic of A. cantonensis,seven female worms were selected to characterize the mi-tochondrial(MT)genomes. Twelve primer pairs based on known MT genome(GQ398121)were used for PCR. The target frag-ments were sequenced and aligned. The gene localization,genome structure,composition of nucleotide,distribution of variable sites,and phylogeny were analyzed by employing multiple softwares. Results Five distinct types were identified from seven com-plete MT genomes. They were similar in size and structure,i.e.,ranging 13 491-13 502 bp,including 12 protein-coding genes,2 ribosomal genes,22 tRNA genes,and 2 major non-coding regions. All the genes were localized at the same strand and had the same transcription direction. A total of 745 variable sites were identified,accounting for 5.5%. Among the variable sites,59 were deletion/insert mutations,105 transversions,and 581 transitions. The variable sites distributed evenly at the complete genome. Conclusion The study reveals the mutation profile in the whole MT genome of A. cantonensis and thus will facilitate the develop-ment of intraspecific differential diagnosis.
ABSTRACT
ABSTRACT:To explore the feasibility of DNA barcoding in the identification of sandflies ,the mitochondrial cytochrome c oxidase subunit I (COI) genes of 9 dominant species in China from three genera in the Phlebotominae were studied .The in‐traspecies and interspecies genetic distances were calculated using the Kimura‐2‐parameter model ,and the phylogenetic trees were reconstructed by neighbor‐joining (NJ) method .Results showed that all of sandflies species were successfully distinguish‐ed by the phylogenetic trees .The average intraspecific genetic distance (0 .8% ) was much less than the average interspecific ge‐netic distance (11 .2% ) .The study based on COI sequences indicates that the sandflies from China could be well distinguished with the commonly used COI barcodes ,which are potentially well utilized in sandflies species identification .
ABSTRACT
Objective To analyze the causes of residual gallbladder disease after claparoscopic cholecystectomy. Methods From Feb-ruary 2006 to February 2013, there were 30 patients with residual gallbladder disease after laparoscopic cholecystectomy, and they were given second surgery in our hospital. Results The residual gallbladder lesions were successfully removed. Symtoms before operation were relieved or disappear. The average duration of hospital stays was 8. 2 d. The difference between before and after surgery was statistically significant(P<0. 05). There were 2 cases of delayed wound healing, and the others were of good curative effect. And blood test indicators of all the pa-tients recovered to normal when they leave hospital. Conclusion The surgery was carried out strictly accordding to the relevant procedure to prevent the occurrence of residual cystic lesions. If the patients were found to have obvious symptoms of residual gallbladder disease,they should be early diagnosed and recieved second surgery as soon as possible so as to cure disease and improve quality of life after surgery.